Capucine Penicaud is Programme Manager for the International Coalition to Eliminate HBV (ICE-HBV). She leads the secretariat of the Coalition based at the Doherty Institute for Infection and Immunity in Melbourne, Australia. She has over ten years of experience in international development and global health, both in non-profit (International AIDS Society) and government organisations (French Foreign Affairs Ministry). Her work focuses on the design and set-up of strategic initiatives such as the IAS’ Towards an HIV Cure programme, ICE-HBV and The Hepatitis Fund.
Capucine Penicaud
Recently Published Protocols
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An Optimized Ex Vivo ELISpot assay to identify IFN gamma positive, HBV-specific T cells in Chronic Hepatitis B patients
During a traditional ELISpot assay, low HBV Specific T cell frequencies have hindered effective ex vivo analysis. We... [Read more]
12 April, 2023 -
Multiplexed immunostaining of formalin-fixed paraffin-embedded liver biopsy tissues with metal-conjugated antibodies for tissue imaging mass cytometry
This protocol describes the approach used to stain formalin-fixed paraffin-embedded liver biopsy slides with a panel of metal-conjugated antibodi... [Read more]12 April, 2023 -
A modified kit-based HBV protein-free DNA extraction from liver tissues and cell cultures for HBV cccDNA Southern blot and qPCR
- cccDNA quantification from total DNA preparation by qPCR with cccDNA-selective primers is challenging because of the coexisting HBV D... [Read more]
21 September, 2022 -
A sensitive and rapid Southern blot assay based on branched DNA technology for the detection of HBV DNA in cell culture and liver tissue samples
- Sothern blot is considered the “gold standard” for cccDNA detection because it can separate the cccDNA from protein-free relaxed circ... [Read more]
21 September, 2022 -
An Optimized Ex Vivo Flurospot assay to identify multi-functional HBV-specific T cells in Chronic Hepatitis B patients
During a traditional FluroSpot assay, low HBV Specific T cell frequencies have hindered effective ex vivo analysis. We overcame this obstacle to ... [Read more]07 March, 2022 -
Fluorescent-bait labelling for the ex vivo detection of HBV antigen-specific B cells
Fluorescently conjugated antigen-bait systems have been extensively used to identify antigen-specific B cells and probe humoral immunity across d... [Read more]30 April, 2021 -
Tetramer enrichment for HBV-specific CD8+ T cells
The frequency of HBV-specific CD8+ T cells can be very low, depending on the specificity. Therefore, they cannot always be detected by convention... [Read more]03 July, 2020 -
A fluorescent in situ hybridization (FISH) assay for detection of HBV DNA in cell culture models
- Although a plethora of knowledge on the molecular life cycle of Hepatitis B Virus has been gained by utilizing classical methods, the... [Read more]
19 June, 2020 -
HBV Ribonuclease H assay resolved by denaturing PAGE
- This assay characterizes the products of HBV RNase H activity on a DNA:RNA heteroduplex.
- Products are resolved by denatur... [Read more]
06 June, 2019 -
HBV Ribonuclease H FRET Assay
- This assay detects the activity of HBV RNaseH using fluorescence resonance energy transfer
- The ribonuclease H (RNaseH) su... [Read more]
06 June, 2019