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Recently Published Protocols
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Tetramer enrichment for HBV-specific CD8+ T cells
The frequency of HBV-specific CD8+ T cells can be very low, depending on the specificity. Therefore, ... [Read more]03 July, 2020 -
A fluorescent in situ hybridization (FISH) assay for detection of HBV DNA in cell culture models
- Although a plethora of knowledge on the molecular life cycle of Hepatitis B Virus has bee... [Read more]
19 June, 2020 -
HBV Ribonuclease H assay resolved by denaturing PAGE
- This assay characterizes the products of HBV RNase H activity on a DNA:RNA heteroduplex.[Read more]
06 June, 2019 -
HBV Ribonuclease H FRET Assay
- This assay detects the activity of HBV RNaseH using fluorescence resonance energy transfer... [Read more]
06 June, 2019 -
A Southern Blot Assay for Detection of HBV cccDNA from Cell Cultures
- As an essential component of HBV life cycle, the viral covalently closed circular DNA (cc... [Read more]
03 June, 2019 -
Modeling HBV infection and therapy in immunodeficient NOD-Rag1-/-IL2RgammaC-null (NRG) fumarylacetoacetate hydrolase (FAH) knockout mice with human chimeric liver
- Besides human, only a few species have been reported to be permissive for Hepatitis B vir... [Read more]
25 May, 2019 -
Hepatitis B Virus Polymerase Epsilon RNA Binding Assays
- HBV polymerase activity is assayed by measuring its ability to bind viral RNA at a second... [Read more]
24 May, 2019 -
In Vitro Hepatitis B Virus Polymerase Priming Assay
- HBV polymerase activity is assayed by measuring the first step in DNA synthesis, protein ... [Read more]
23 May, 2019 -
HBV and HDV infection in uPA/SCID mice with humanized livers
- This protocol describes the infection of liver humanized mice (here UPA/SCID mice) with hep... [Read more]
21 May, 2019 -
Evaluating mouse HBV-specific CD8+ T cell responses by ELISPOT assay
- Immunocompetent mouse models that allow for the investigation of virus replication and the ... [Read more]
21 May, 2019