HBV Research Protocols

There is an urgent need for centralized repositories of HBV-related materials that are readily accessible to HBV researchers globally. Critical to this will be quality assurance of the samples, and the availability of matching clinical data. This repository of HBV-related research protocols is designed to facilitate studies and the development of new drugs.

This project is designed to complement the upcoming NIAID reagents repository by making corresponding quality-controlled research protocols available freely for all researchers around the world.

When citing protocols from this database, please cite the original publications from which these protocols have been adapted. The origin of these original publications can be found within the protocols. Please also acknowledge this ICE-HBV Protocols Database. Any questions can be directed to info@ice-hbv.org

The review of these protocols has been led by Haitao Guo and the ICE-HBV working group members including Lena Allweiss, Maura Dandri, Jianming Hu, Jake Liang, Margaret Littlejohn, Peter Revill, and Barbara Testoni.

The development of the database is coordinated by Marley Easterbrook.

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A fluorescent in situ hybridization (FISH) assay for detection of HBV DNA in cell culture models

Xiaonan Zhang, Lei Yue, Chang Li, Zhenghong Yuan

Shanghai Public Health Clinical Center, Key Lab of Medical Molecular Virology, Shanghai Medical College, Fudan University.
Cell Cultures HBV Nucleic Acid Analyses
  • Although a plethora of knowledge on the molecular life cycle of Hepatitis B Virus has been gained by utilizing classical methods, they can only inform on the average level of the tested molecule without subcellular or histological con... [Read more]

HBV and HDV infection in uPA/SCID mice with humanized livers

Volz, T. 1; Lütgehetmann, M. 1,3; Dandri, M. 1,2

1. Medical Clinic, University Medic al Center Hamburg-Eppendorf, Hamburg, Germany, 2. German Centre for Infection Research (DZIF), Munich, Hamburg and Heidelberg partner sites, Germany, 3. Institute of Microbiology, Virology and Hygiene, University Medical Center Hamburg-Eppendorf, Hamburg, Germany
Animal Models HBV Nucleic Acid Analyses
  • This protocol describes the infection of liver humanized mice (here UPA/SCID mice) with hepatitis B and /or hepatitis D viruses.
  • We also address the characteristics of infection kinetics and the factors influencing viral spread in humanized mouse livers.[Read more]

Detection and characterisation of integrated Hepatitis B virus DNA using inverse nested PCR

Dr. Thomas Tu

Department of Infectious Diseases, Molecular Virology, University Hospital Heidelberg, Heidelberg, Germany
Cell Cultures HBV Nucleic Acid Analyses In Vitro HBV Infection Systems
  • The integration of HBV DNA into the host cell genome can occur during HBV infection, though it does not produce new infectious virus particles.
  • HBV DNA integration is one possible cause of HBV-induced liver cancer and could b... [Read more]