HBV Research Protocols

There is an urgent need for centralized repositories of HBV-related materials that are readily accessible to HBV researchers globally. Critical to this will be quality assurance of the samples, and the availability of matching clinical data. This repository of HBV-related research protocols is designed to facilitate studies and the development of new drugs.

This project is designed to complement the upcoming NIAID reagents repository by making corresponding quality-controlled research protocols available freely for all researchers around the world.

When citing protocols from this database, please cite the original publications from which these protocols have been adapted. The origin of these original publications can be found within the protocols. Please also acknowledge this ICE-HBV Protocols Database. Any questions can be directed to info@ice-hbv.org

The review of these protocols has been led by Haitao Guo and the ICE-HBV working group members including Lena Allweiss, Maura Dandri, Jianming Hu, Jake Liang, Margaret Littlejohn, Peter Revill, and Barbara Testoni.

The development of the database is coordinated by Marley Easterbrook.

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An Optimized Ex Vivo ELISpot assay to identify IFN gamma positive, HBV-specific T cells in Chronic Hepatitis B patients

Conan Chua, Aman Mehrotra and Dr. Adam Gehring

Toronto Center for Liver Disease
Cell Cultures Immunology Assays

During a traditional ELISpot assay, low HBV Specific T cell frequencies have hindered effective ex vivo analysis. We overcame this obstacle to measure ex vivo T cell responses in CHB patients, by modifying the key variables of cell number and the peptide pulsing method to improve ex vivo detection of HBV-specific T cells.

An Optimized Ex Vivo Flurospot assay to identify multi-functional HBV-specific T cells in Chronic Hepatitis B patients

Conan Chua, Aman Mehrotra, Dr. Adam Gehring

Toronto Centre for Liver Disease, Toronto, Ontario, Canada
Cell Cultures Immunology Assays
During a traditional FluroSpot assay, low HBV Specific T cell frequencies have hindered effective ex vivo analysis. We overcame this obstacle to measure ex vivo T cell responses in CHB patients, by modifying the key variables of cell number and the peptide pulsing method to improve ex vivo detection of HBV-specific T cells.

A fluorescent in situ hybridization (FISH) assay for detection of HBV DNA in cell culture models

Xiaonan Zhang, Lei Yue, Chang Li, Zhenghong Yuan

Shanghai Public Health Clinical Center, Key Lab of Medical Molecular Virology, Shanghai Medical College, Fudan University.
Cell Cultures HBV Nucleic Acid Analyses
  • Although a plethora of knowledge on the molecular life cycle of Hepatitis B Virus has been gained by utilizing classical methods, they can only inform on the average level of the tested molecule without subcellular or histological con... [Read more]

Stem Cell Derived Hepatocyte-Like Cells for Hepatitis B Virus Infection

Yuchen Xia (1), Seung Bum Park (2), T. Jake Liang (2)

1. Wuhan University, Wuhan, China 2. National Institutes of Health, Bethesda, USA
Cell Cultures In Vitro HBV Infection Systems
  • Human pluripotent stem cells derived hepatocyte-like cells (HLCs) are capable of expressing hepatocyte markers and host factors important for hepatic function maintenance.
  • These cells fully support HBV infection and virus-host interactions.
  • Stem cell-derived HLCs provide a new tool for antiviral drug screening and development.

Detection and characterisation of integrated Hepatitis B virus DNA using inverse nested PCR

Dr. Thomas Tu

Department of Infectious Diseases, Molecular Virology, University Hospital Heidelberg, Heidelberg, Germany
Cell Cultures HBV Nucleic Acid Analyses In Vitro HBV Infection Systems
  • The integration of HBV DNA into the host cell genome can occur during HBV infection, though it does not produce new infectious virus particles.
  • HBV DNA integration is one possible cause of HBV-induced liver cancer and could b... [Read more]