Financing prevention, testing and treatment of hepatitis in the context of Universal Health Coverage
Recently Published Protocols
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A modified kit-based HBV protein-free DNA extraction from liver tissues and cell cultures for HBV cccDNA Southern blot and qPCR
- cccDNA quantification from total DNA preparation by qPCR with c... [Read more]
21 September, 2022 -
A sensitive and rapid Southern blot assay based on branched DNA technology for the detection of HBV DNA in cell culture and liver tissue samples
- Sothern blot is considered the “gold standard” for cccDNA detec... [Read more]
21 September, 2022 -
An Optimized Ex Vivo Flurospot assay to identify multi-functional HBV-specific T cells in Chronic Hepatitis B patients
During a traditional FluroSpot assay, low HBV Specific T cell frequencies h... [Read more]07 March, 2022 -
Fluorescent-bait labelling for the ex vivo detection of HBV antigen-specific B cells
Fluorescently conjugated antigen-bait systems have been extensively used to... [Read more]30 April, 2021 -
Tetramer enrichment for HBV-specific CD8+ T cells
The frequency of HBV-specific CD8+ T cells can be very low, depending on th... [Read more]03 July, 2020 -
A fluorescent in situ hybridization (FISH) assay for detection of HBV DNA in cell culture models
- Although a plethora of knowledge on the molecular life cycle of... [Read more]
19 June, 2020 -
HBV Ribonuclease H assay resolved by denaturing PAGE
- This assay characterizes the products of HBV RNase H activity on... [Read more]
06 June, 2019 -
HBV Ribonuclease H FRET Assay
- This assay detects the activity of HBV RNaseH using fluorescence... [Read more]
06 June, 2019 -
A Southern Blot Assay for Detection of HBV cccDNA from Cell Cultures
- As an essential component of HBV life cycle, the viral covalent... [Read more]
03 June, 2019 -
Modeling HBV infection and therapy in immunodeficient NOD-Rag1-/-IL2RgammaC-null (NRG) fumarylacetoacetate hydrolase (FAH) knockout mice with human chimeric liver
- Besides human, only a few species have been reported to be perm... [Read more]
25 May, 2019