HBV Research Protocols

There is an urgent need for centralized repositories of HBV-related materials that are readily accessible to HBV researchers globally. Critical to this will be quality assurance of the samples, and the availability of matching clinical data. This repository of HBV-related research protocols is designed to facilitate studies and the development of new drugs.

This project is designed to complement the upcoming NIAID reagents repository by making corresponding quality-controlled research protocols available freely for all researchers around the world.

When citing protocols from this database, please cite the original publications from which these protocols have been adapted. The origin of these original publications can be found within the protocols. Please also acknowledge this ICE-HBV Protocols Database. Any questions can be directed to info@ice-hbv.org

The review of these protocols has been led by Haitao Guo and the ICE-HBV working group members including Lena Allweiss, Maura Dandri, Jianming Hu, Jake Liang, Margaret Littlejohn, Peter Revill, and Barbara Testoni.

The development of the database is coordinated by Marley Easterbrook.

Modeling HBV infection and therapy in immunodeficient NOD-Rag1-/-IL2RgammaC-null (NRG) fumarylacetoacetate hydrolase (FAH) knockout mice with human chimeric liver

James Ahodantin (1), Feng Li (2), and Lishan Su (1)

(1) Lineberger Comprehensive Cancer Center, Department of microbiology and immunology, school of medicine, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA. (2) Guangzhou Eighth People’s Hospital, Guangzhou Medical University, Guangzhou 510060, Guangdong, China. *Correspondence: Correspondence should be addressed to L.S. (lsu@med.unc.edu), telephone: 919-966-6654; fax: 919-966-8212
  • Besides human, only a few species have been reported to be permissive for Hepatitis B virus (HBV) infection(1-3). Human liver chimeric mouse models have been developed to ov... [Read more]

HBV and HDV infection in uPA/SCID mice with humanized livers

Volz, T. 1; Lütgehetmann, M. 1,3; Dandri, M. 1,2

1. Medical Clinic, University Medic al Center Hamburg-Eppendorf, Hamburg, Germany, 2. German Centre for Infection Research (DZIF), Munich, Hamburg and Heidelberg partner sites, Germany, 3. Institute of Microbiology, Virology and Hygiene, University Medical Center Hamburg-Eppendorf, Hamburg, Germany
  • This protocol describes the infection of liver humanized mice (here UPA/SCID mice) with hepatitis B and /or hepatitis D viruses.
  • We also address the characteristics of infection kinetics and the factors influencing viral spread in humanized mouse livers.
  • ... [Read more]

Hydrodynamic HBV transfection in mouse models

Li-Ling Wu 1,2, Hurng-Yi Wang 1, and Pei-Jer Chen 1,3,4

1. Graduate Institute of Clinical Medicine, 2. Department and Institute of Physiology, School of Medicine, National Yang-Ming University, 3. Hepatitis Research Center, and 4. Department of Medical Research, National Taiwan University College of Medicine and National Taiwan University Hospital, Taipei, Taiwan.
  • The method of hydrodynamic delivery (1, 2) was used to establish a mouse model of hepatitis B virus persistence by transfecting hepatocytes in vivo with HBV genome expressing viral antigens and replicative intermediates, resulting in production of viral particles (3).
  • ... [Read more]

Immunofluorescent Staining for the Hepatitis B Core Antigen in frozen Liver Sections

Lena Allweiss, Marc Lütgehetmann, Maura Dandri

I. Deptartment of Internal Medicine, University Medical Center Hamburg-Eppendorf, Hamburg, Germany
  • The HBV core antigen (HBcAg) is the structural protein of the viral nucleocapsids but is also recruited to the nuclear HBV persistence form. Thus, HBcAg can be detected in the cytoplasm and nucleus of infected hepatocytes.
  • Here, we r... [Read more]