Massimo Levrero

Board Member

Rome, Italy

Tetramer enrichment for HBV-specific CD8+ T cells

The frequency of HBV-specific CD8+ T cells can be very low, depending on the specificity. Therefore, they cannot always be detected by conventional e... [Read more]

03 July, 2020

A fluorescent in situ hybridization (FISH) assay for detection of HBV DNA in cell culture models

Although a plethora of knowledge on the molecular life cycle of Hepatitis B Virus has been gained by utilizing classical methods, they can on... [Read more]

19 June, 2020

HBV Ribonuclease H assay resolved by denaturing PAGE

This assay characterizes the products of HBV RNase H activity on a DNA:RNA heteroduplex.

Products are resolved by denaturing PAGE... [Read more]

06 June, 2019

HBV Ribonuclease H FRET Assay

This assay detects the activity of HBV RNaseH using fluorescence resonance energy transfer

The ribonuclease H (RNaseH) substrate ... [Read more]

06 June, 2019

A Southern Blot Assay for Detection of HBV cccDNA from Cell Cultures

As an essential component of HBV life cycle, the viral covalently closed circular DNA (cccDNA) is synthesized and maintained at low copy numb... [Read more]

03 June, 2019

Modeling HBV infection and therapy in immunodeficient NOD-Rag1-/-IL2RgammaC-null (NRG) fumarylacetoacetate hydrolase (FAH) knockout mice with human chimeric liver

Besides human, only a few species have been reported to be permissive for Hepatitis B virus (HBV) infection(1-3). Human liver chimeric mouse ... [Read more]

25 May, 2019

Hepatitis B Virus Polymerase Epsilon RNA Binding Assays

HBV polymerase activity is assayed by measuring its ability to bind viral RNA at a secondary-structure motif, the epsilon (e) stem-loop struc... [Read more]

24 May, 2019

In Vitro Hepatitis B Virus Polymerase Priming Assay

HBV polymerase activity is assayed by measuring the first step in DNA synthesis, protein priming, wherein the polymerase becomes nucleotide-l... [Read more]

23 May, 2019

HBV and HDV infection in uPA/SCID mice with humanized livers

This protocol describes the infection of liver humanized mice (here UPA/SCID mice) with hepatitis B and /or hepatitis D viruses.

We a... [Read more]

21 May, 2019

Evaluating mouse HBV-specific CD8+ T cell responses by ELISPOT assay

Immunocompetent mouse models that allow for the investigation of virus replication and the immune response to HBV include hydrodynamic injectio... [Read more]

21 May, 2019

Governing Board

Massimo Levrero

Board Member

Rome, Italy

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