Massimo Levrero

Board Member

Rome, Italy

READ MORE ABOUT

HBV Ribonuclease H assay resolved by denaturing PAGE
- This assay characterizes the products of HBV RNase H activity on a DNA:RNA heteroduplex.
- Products are resolved by denaturing PAGE... [Read more]
06 June, 2019
HBV Ribonuclease H FRET Assay
- This assay detects the activity of HBV RNaseH using fluorescence resonance energy transfer
- The ribonuclease H (RNaseH) substrate ... [Read more]
06 June, 2019
A Southern Blot Assay for Detection of HBV cccDNA from Cell Cultures
- As an essential component of HBV life cycle, the viral covalently closed circular DNA (cccDNA) is synthesized and maintained at low copy numb... [Read more]
03 June, 2019
Modeling HBV infection and therapy in immunodeficient NOD-Rag1-/-IL2RgammaC-null (NRG) fumarylacetoacetate hydrolase (FAH) knockout mice with human chimeric liver
- Besides human, only a few species have been reported to be permissive for Hepatitis B virus (HBV) infection(1-3). Human liver chimeric mouse ... [Read more]
25 May, 2019
Hepatitis B Virus Polymerase Epsilon RNA Binding Assays
- HBV polymerase activity is assayed by measuring its ability to bind viral RNA at a secondary-structure motif, the epsilon (e) stem-loop struc... [Read more]
24 May, 2019
In Vitro Hepatitis B Virus Polymerase Priming Assay
- HBV polymerase activity is assayed by measuring the first step in DNA synthesis, protein priming, wherein the polymerase becomes nucleotide-l... [Read more]
23 May, 2019
HBV and HDV infection in uPA/SCID mice with humanized livers
- This protocol describes the infection of liver humanized mice (here UPA/SCID mice) with hepatitis B and /or hepatitis D viruses.
- We a... [Read more]
21 May, 2019
Evaluating mouse HBV-specific CD8+ T cell responses by ELISPOT assay
- Immunocompetent mouse models that allow for the investigation of virus replication and the immune response to HBV include hydrodynamic injectio... [Read more]
21 May, 2019
HBV RNA detection in liver tissues by in situ hybridization
- HBV RNA detection at the cellular level in the liver
- Multiplex detection of two RNA ... [Read more]
21 May, 2019
Hydrodynamic HBV transfection in mouse models
- The method of hydrodynamic delivery (1, 2) was used to establish a mouse model of hepatitis B virus persistence by transfecting hepatocytes <... [Read more]
13 May, 2019

Governing Board

Massimo Levrero

Board Member

Rome, Italy

READ MORE ABOUT

Conference on Liver Disease in Africa 2020 (COLDA)

(Postponed) Public Forum at the International HBV Meeting (Free Entry)

ICE-HBV Workshop On Serum Biomarkers