Global HDV Elimination: Challenges and Opportunities Webinar – July 20th, 2023

During a traditional ELISpot assay, low HBV Specific T cell frequencies have hindered effective ex vivo analysis. We overcame this obstacle... [Read more]

This protocol describes the approach used to stain formalin-fixed paraffin-embedded liver biopsy slides with a panel of metal-conjugated antibodies for subsequent analy... [Read more]

• cccDNA quantification from total DNA preparation by qPCR with cccDNA-selective primers is challenging because of the coexisting HBV DNA forms (replicative i... [Read more]

• Sothern blot is considered the “gold standard” for cccDNA detection because it can separate the cccDNA from protein-free relaxed circular DNA (pf-rcDNA, aka... [Read more]

During a traditional FluroSpot assay, low HBV Specific T cell frequencies have hindered effective ex vivo analysis. We overcame this obstacle to measure ex vivo T cell ... [Read more]

Fluorescently conjugated antigen-bait systems have been extensively used to identify antigen-specific B cells and probe humoral immunity across different settings. Usin... [Read more]

The frequency of HBV-specific CD8+ T cells can be very low, depending on the specificity. Therefore, they cannot always be detected by conventional ex vivo tet... [Read more]

• Although a plethora of knowledge on the molecular life cycle of Hepatitis B Virus has been gained by utilizing classical methods, they can only inform on th... [Read more]

• This assay characterizes the products of HBV RNase H activity on a DNA:RNA heteroduplex.
• Products are resolved by denaturing PAGE to assess specificity of cleavage and effects of inhibitory compounds on the enzyme

• This assay detects the activity of HBV RNaseH using fluorescence resonance energy transfer
• The ribonuclease H (RNaseH) substrate is an RNA DNA h... [Read more]