Sign up for news

Add your name and email address for us to add you to our mailing list.

An Optimized Ex Vivo Flurospot assay to identify multi-functional HBV-specific T cells in Chronic Hepatitis B patients

During a traditional FluroSpot assay, low HBV Specific T cell frequencies have hindered effective ex vivo analysis. We overcame this obstacle to measure ex vi... [Read more]

07 March, 2022
Fluorescent-bait labelling for the ex vivo detection of HBV antigen-specific B cells

Fluorescently conjugated antigen-bait systems have been extensively used to identify antigen-specific B cells and probe humoral immunity across different sett... [Read more]

30 April, 2021
Tetramer enrichment for HBV-specific CD8+ T cells

The frequency of HBV-specific CD8+ T cells can be very low, depending on the specificity. Therefore, they cannot always be detected by conventional ex viv... [Read more]

03 July, 2020

A fluorescent in situ hybridization (FISH) assay for detection of HBV DNA in cell culture models

Although a plethora of knowledge on the molecular life cycle of Hepatitis B Virus has been gained by utilizing classical methods, they can only in... [Read more]

19 June, 2020

HBV Ribonuclease H assay resolved by denaturing PAGE

This assay characterizes the products of HBV RNase H activity on a DNA:RNA heteroduplex.

Products are resolved by denaturing PAGE to a... [Read more]

06 June, 2019

HBV Ribonuclease H FRET Assay

This assay detects the activity of HBV RNaseH using fluorescence resonance energy transfer

The ribonuclease H (RNaseH) substrate is an... [Read more]

06 June, 2019

A Southern Blot Assay for Detection of HBV cccDNA from Cell Cultures

As an essential component of HBV life cycle, the viral covalently closed circular DNA (cccDNA) is synthesized and maintained at low copy numbers i... [Read more]

03 June, 2019

Modeling HBV infection and therapy in immunodeficient NOD-Rag1-/-IL2RgammaC-null (NRG) fumarylacetoacetate hydrolase (FAH) knockout mice with human chimeric liver

Besides human, only a few species have been reported to be permissive for Hepatitis B virus (HBV) infection(1-3). Human liver chimeric mouse model... [Read more]

25 May, 2019

Hepatitis B Virus Polymerase Epsilon RNA Binding Assays

HBV polymerase activity is assayed by measuring its ability to bind viral RNA at a secondary-structure motif, the epsilon (e) stem-loop structure.... [Read more]

24 May, 2019

In Vitro Hepatitis B Virus Polymerase Priming Assay

HBV polymerase activity is assayed by measuring the first step in DNA synthesis, protein priming, wherein the polymerase becomes nucleotide-labell... [Read more]

23 May, 2019