HBV Elimination 101

ICE-HBV, The Hepatitis B Foundation and the World Hepatitis Alliance came together with many other stakeholders and agreed on the shortest route to HBV elimination. Check out their briefing here and use this in your activities with policy makers and lay audiences.

An Optimized Ex Vivo ELISpot assay to identify IFN gamma positive, HBV-specific T cells in Chronic Hepatitis B patients

During a traditional ELISpot assay, low HBV Specific T cell frequencies have hindered effective ex vivo analysis. We overcame ... [Read more]

12 April, 2023
Multiplexed immunostaining of formalin-fixed paraffin-embedded liver biopsy tissues with metal-conjugated antibodies for tissue imaging mass cytometry

This protocol describes the approach used to stain formalin-fixed paraffin-embedded liver biopsy slides with a panel of metal-conjugated antibodies for sub... [Read more]

12 April, 2023
A modified kit-based HBV protein-free DNA extraction from liver tissues and cell cultures for HBV cccDNA Southern blot and qPCR
- cccDNA quantification from total DNA preparation by qPCR with cccDNA-selective primers is challenging because of the coexisting HBV DNA forms (... [Read more]
21 September, 2022
A sensitive and rapid Southern blot assay based on branched DNA technology for the detection of HBV DNA in cell culture and liver tissue samples
- Sothern blot is considered the “gold standard” for cccDNA detection because it can separate the cccDNA from protein-free relaxed circular DNA (... [Read more]
21 September, 2022
An Optimized Ex Vivo Flurospot assay to identify multi-functional HBV-specific T cells in Chronic Hepatitis B patients

During a traditional FluroSpot assay, low HBV Specific T cell frequencies have hindered effective ex vivo analysis. We overcame this obstacle to measure ex... [Read more]

07 March, 2022
Fluorescent-bait labelling for the ex vivo detection of HBV antigen-specific B cells

Fluorescently conjugated antigen-bait systems have been extensively used to identify antigen-specific B cells and probe humoral immunity across different s... [Read more]

30 April, 2021
Tetramer enrichment for HBV-specific CD8+ T cells

The frequency of HBV-specific CD8+ T cells can be very low, depending on the specificity. Therefore, they cannot always be detected by conventional ex ... [Read more]

03 July, 2020

A fluorescent in situ hybridization (FISH) assay for detection of HBV DNA in cell culture models

Although a plethora of knowledge on the molecular life cycle of Hepatitis B Virus has been gained by utilizing classical methods, they can only... [Read more]

19 June, 2020

HBV Ribonuclease H assay resolved by denaturing PAGE

This assay characterizes the products of HBV RNase H activity on a DNA:RNA heteroduplex.

Products are resolved by denaturing PAGE to assess specificity of cleavage and effects of inhibitory compounds on the enzyme

06 June, 2019

HBV Ribonuclease H FRET Assay

This assay detects the activity of HBV RNaseH using fluorescence resonance energy transfer

The ribonuclease H (RNaseH) substrate is... [Read more]

06 June, 2019

HBV Elimination 101

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