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ICE-HBV will be largely present at HepDart 2017
Recently Published Protocols
Tetramer enrichment for HBV-specific CD8+ T cells
The frequency of HBV-specific CD8+ T cells can be very low, depending on the specificity. Therefore, they cannot always be detected by conventional
03 July, 2020
A fluorescent in situ hybridization (FISH) assay for detection of HBV DNA in cell culture models
Although a plethora of knowledge on the molecular life cycle of Hepatitis B Virus has been gained by utilizing classical methods, they can only...
19 June, 2020
HBV Ribonuclease H assay resolved by denaturing PAGE
This assay characterizes the products of HBV RNase H activity on a DNA:RNA heteroduplex.
Products are resolved by denaturing PAGE t...
06 June, 2019
HBV Ribonuclease H FRET Assay
This assay detects the activity of HBV RNaseH using fluorescence resonance energy transfer
The ribonuclease H (RNaseH) substrate is...
06 June, 2019
A Southern Blot Assay for Detection of HBV cccDNA from Cell Cultures
As an essential component of HBV life cycle, the viral covalently closed circular DNA (cccDNA) is synthesized and maintained at low copy number...
03 June, 2019
Modeling HBV infection and therapy in immunodeficient NOD-Rag1-/-IL2RgammaC-null (NRG) fumarylacetoacetate hydrolase (FAH) knockout mice with human chimeric liver
Besides human, only a few species have been reported to be permissive for Hepatitis B virus (HBV) infection(1-3). Human liver chimeric mouse mo...
25 May, 2019
Hepatitis B Virus Polymerase Epsilon RNA Binding Assays
HBV polymerase activity is assayed by measuring its ability to bind viral RNA at a secondary-structure motif, the epsilon (e) stem-loop structu...
24 May, 2019
In Vitro Hepatitis B Virus Polymerase Priming Assay
HBV polymerase activity is assayed by measuring the first step in DNA synthesis, protein priming, wherein the polymerase becomes nucleotide-lab...
23 May, 2019
HBV and HDV infection in uPA/SCID mice with humanized livers
This protocol describes the infection of liver humanized mice (here UPA/SCID mice) with hepatitis B and /or hepatitis D viruses.
21 May, 2019
Evaluating mouse HBV-specific CD8+ T cell responses by ELISPOT assay
Immunocompetent mouse models that allow for the investigation of virus replication and the immune response to HBV include hydrodynamic injection,...
21 May, 2019