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HBV Research Protocols

Fluorescent-bait labelling for the ex vivo detection of HBV antigen-specific B cells

Fluorescently conjugated antigen-bait systems have been extensively used to identify antigen-specific B cells and probe humoral immunity across different settings. Usin... [Read more]

30 April, 2021
Tetramer enrichment for HBV-specific CD8+ T cells

The frequency of HBV-specific CD8+ T cells can be very low, depending on the specificity. Therefore, they cannot always be detected by conventional ex vivo tet... [Read more]

03 July, 2020
A fluorescent in situ hybridization (FISH) assay for detection of HBV DNA in cell culture models
- Although a plethora of knowledge on the molecular life cycle of Hepatitis B Virus has been gained by utilizing classical methods, they can only inform on th... [Read more]
19 June, 2020
HBV Ribonuclease H assay resolved by denaturing PAGE
- This assay characterizes the products of HBV RNase H activity on a DNA:RNA heteroduplex.
- Products are resolved by denaturing PAGE to assess spec... [Read more]
06 June, 2019
HBV Ribonuclease H FRET Assay
- This assay detects the activity of HBV RNaseH using fluorescence resonance energy transfer
- The ribonuclease H (RNaseH) substrate is an RNA DNA h... [Read more]
06 June, 2019
A Southern Blot Assay for Detection of HBV cccDNA from Cell Cultures
- As an essential component of HBV life cycle, the viral covalently closed circular DNA (cccDNA) is synthesized and maintained at low copy numbers in the nucl... [Read more]
03 June, 2019
Modeling HBV infection and therapy in immunodeficient NOD-Rag1-/-IL2RgammaC-null (NRG) fumarylacetoacetate hydrolase (FAH) knockout mice with human chimeric liver
- Besides human, only a few species have been reported to be permissive for Hepatitis B virus (HBV) infection(1-3). Human liver chimeric mouse models have bee... [Read more]
25 May, 2019
Hepatitis B Virus Polymerase Epsilon RNA Binding Assays
- HBV polymerase activity is assayed by measuring its ability to bind viral RNA at a secondary-structure motif, the epsilon (e) stem-loop structure.
24 May, 2019
In Vitro Hepatitis B Virus Polymerase Priming Assay
- HBV polymerase activity is assayed by measuring the first step in DNA synthesis, protein priming, wherein the polymerase becomes nucleotide-labelled.
23 May, 2019
HBV and HDV infection in uPA/SCID mice with humanized livers
- This protocol describes the infection of liver humanized mice (here UPA/SCID mice) with hepatitis B and /or hepatitis D viruses.
- We also address the... [Read more]
21 May, 2019

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EASLnews @EASLnews·

14 Oct

🚌 All aboard the bus to savouring science at #ILC2022 in London!

Make your science/R&D findings part of the vibrant programme. 𝗦𝘂𝗯𝗺𝗶𝘁 𝘆𝗼𝘂𝗿 𝗮𝗯𝘀𝘁𝗿𝗮𝗰𝘁 by 23 Nov.

Explore the abstract categories & submit on http://easl.eu/ILC2022-abstract

@AleksanderKrag @ThomasBerg24

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Joost PH Drenth @joostphdrenth·

26 Oct

@ESPGHANSociety @ESPGHANelearn published a new advice guide on the treatment of the reactivation of hepatitis B virus in children

Great infographic !
@EASLnews @my_ueg @ICE_HBV @VIRCAN_ca @CHIPO_HBV @GastroRTs

https://espghan.info/files/Reactivation%20of%20Hepatitis%20B%20Virus%20(HBV).%20ESPGHAN%20Advice%20Guide.%202020.%20Ver1..pdf

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