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HBV Research Protocols

Tetramer enrichment for HBV-specific CD8+ T cells

The frequency of HBV-specific CD8+ T cells can be very low, depending on the specificity. Therefore, they cannot always be detected by conventional ex vivo tet... [Read more]

03 July, 2020
A fluorescent in situ hybridization (FISH) assay for detection of HBV DNA in cell culture models
- Although a plethora of knowledge on the molecular life cycle of Hepatitis B Virus has been gained by utilizing classical methods, they can only inform on th... [Read more]
19 June, 2020
HBV Ribonuclease H assay resolved by denaturing PAGE
- This assay characterizes the products of HBV RNase H activity on a DNA:RNA heteroduplex.
- Products are resolved by denaturing PAGE to assess spec... [Read more]
06 June, 2019
HBV Ribonuclease H FRET Assay
- This assay detects the activity of HBV RNaseH using fluorescence resonance energy transfer
- The ribonuclease H (RNaseH) substrate is an RNA DNA h... [Read more]
06 June, 2019
A Southern Blot Assay for Detection of HBV cccDNA from Cell Cultures
- As an essential component of HBV life cycle, the viral covalently closed circular DNA (cccDNA) is synthesized and maintained at low copy numbers in the nucl... [Read more]
03 June, 2019
Modeling HBV infection and therapy in immunodeficient NOD-Rag1-/-IL2RgammaC-null (NRG) fumarylacetoacetate hydrolase (FAH) knockout mice with human chimeric liver
- Besides human, only a few species have been reported to be permissive for Hepatitis B virus (HBV) infection(1-3). Human liver chimeric mouse models have bee... [Read more]
25 May, 2019
Hepatitis B Virus Polymerase Epsilon RNA Binding Assays
- HBV polymerase activity is assayed by measuring its ability to bind viral RNA at a secondary-structure motif, the epsilon (e) stem-loop structure.
24 May, 2019
In Vitro Hepatitis B Virus Polymerase Priming Assay
- HBV polymerase activity is assayed by measuring the first step in DNA synthesis, protein priming, wherein the polymerase becomes nucleotide-labelled.
23 May, 2019
HBV and HDV infection in uPA/SCID mice with humanized livers
- This protocol describes the infection of liver humanized mice (here UPA/SCID mice) with hepatitis B and /or hepatitis D viruses.
- We also address the... [Read more]
21 May, 2019
Evaluating mouse HBV-specific CD8+ T cell responses by ELISPOT assay
- Immunocompetent mouse models that allow for the investigation of virus replication and the immune response to HBV include hydrodynamic injection, HBV genome t... [Read more]
21 May, 2019

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Antons Mozalevskis @rixwolff·

9h

Many low- and middle- income countries have improved access to life-saving #HepatitisC medicines with prices as low as US$60 for three months curative treatment - according to @WHO report just published today: https://www.who.int/news/item/27-01-2021-who-highlights-progress-in-accelerating-access-to-hepatitis-c-diagnostics-and-treatment-in-low-and-middle-income-countries

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MedicinesPatentPool @MedsPatentPool·

13h

#NowSpeaking Françoise Renaud @WHO: “Non-inclusion in #VoluntaryLicensing agreements is a major obstacle” for #affordability of direct acting antivirals for #HepC

#EndHepatitis 2

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