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HBV Research Protocols

Fluorescent-bait labelling for the ex vivo detection of HBV antigen-specific B cells

Fluorescently conjugated antigen-bait systems have been extensively used to identify antigen-specific B cells and probe humoral immunity across different settings. Usin... [Read more]

30 April, 2021
Tetramer enrichment for HBV-specific CD8+ T cells

The frequency of HBV-specific CD8+ T cells can be very low, depending on the specificity. Therefore, they cannot always be detected by conventional ex vivo tet... [Read more]

03 July, 2020
A fluorescent in situ hybridization (FISH) assay for detection of HBV DNA in cell culture models
- Although a plethora of knowledge on the molecular life cycle of Hepatitis B Virus has been gained by utilizing classical methods, they can only inform on th... [Read more]
19 June, 2020
HBV Ribonuclease H assay resolved by denaturing PAGE
- This assay characterizes the products of HBV RNase H activity on a DNA:RNA heteroduplex.
- Products are resolved by denaturing PAGE to assess spec... [Read more]
06 June, 2019
HBV Ribonuclease H FRET Assay
- This assay detects the activity of HBV RNaseH using fluorescence resonance energy transfer
- The ribonuclease H (RNaseH) substrate is an RNA DNA h... [Read more]
06 June, 2019
A Southern Blot Assay for Detection of HBV cccDNA from Cell Cultures
- As an essential component of HBV life cycle, the viral covalently closed circular DNA (cccDNA) is synthesized and maintained at low copy numbers in the nucl... [Read more]
03 June, 2019
Modeling HBV infection and therapy in immunodeficient NOD-Rag1-/-IL2RgammaC-null (NRG) fumarylacetoacetate hydrolase (FAH) knockout mice with human chimeric liver
- Besides human, only a few species have been reported to be permissive for Hepatitis B virus (HBV) infection(1-3). Human liver chimeric mouse models have bee... [Read more]
25 May, 2019
Hepatitis B Virus Polymerase Epsilon RNA Binding Assays
- HBV polymerase activity is assayed by measuring its ability to bind viral RNA at a secondary-structure motif, the epsilon (e) stem-loop structure.
24 May, 2019
In Vitro Hepatitis B Virus Polymerase Priming Assay
- HBV polymerase activity is assayed by measuring the first step in DNA synthesis, protein priming, wherein the polymerase becomes nucleotide-labelled.
23 May, 2019
HBV and HDV infection in uPA/SCID mice with humanized livers
- This protocol describes the infection of liver humanized mice (here UPA/SCID mice) with hepatitis B and /or hepatitis D viruses.
- We also address the... [Read more]
21 May, 2019

ICE-HBV TwitterFollow

Retweet on Twitter ICE-HBV Retweeted

Hepatitis Australia @HepAus·

16h

Thank you Minister for Health @GregHuntMP for your inspiring #WorldHepatitisDay 2021 message:

“Our task is to work with you on a simple goal: eliminating hepatitis as a public health threat in Australia, helping to save every life we can.”

Full video: https://www.youtube.com/watch?v=rIeF7_XlV6g

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Retweet on Twitter ICE-HBV Retweeted

ELPA 🇪🇺🇺🇳@EuropeLiver·

29 Jul

Meet the Principal Investigator of the @ipcureb Cure B project
Dr. Fabien Zoulim and ELPA president @Marko_Korenjak
@Inserm #hepatitisB #Cure #WorldHepatitisDay #LiverCancer #EndHep @HorizonEU @EU_Health
@hans_kluge @SKyriakidesEU
@rixwolff @NO_hep
https://www.youtube.com/watch?v=iqJeIAUjyxg 2

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