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Author: admin

Abstract submission for ANRS HBV Cure workshop is open until April 21, 2017

POSTED ON: 15 Mar, 2017

 

Apply here.

Please note that the presentations are available as ‘posters’ only.

Deadlines:

Deadline for abstract submission: April 21st, 2017
Notification of acceptance:April 28th, 2017

 

 

Categories ANRS, Workshop

HBV Research Protocols

  • An Optimized Ex Vivo ELISpot assay to identify IFN gamma positive, HBV-specific T cells in Chronic Hepatitis B patients

    During a traditional ELISpot assay, low HBV Specific T cell frequencies have hindered effective ex vivo analysis. We overcame this obstacle to measure ex vivo T cell ... [Read more]

    12 April, 2023
  • Multiplexed immunostaining of formalin-fixed paraffin-embedded liver biopsy tissues with metal-conjugated antibodies for tissue imaging mass cytometry

    This protocol describes the approach used to stain formalin-fixed paraffin-embedded liver biopsy slides with a panel of metal-conjugated antibodies for subsequent analysis by Standard BioTool Hyp... [Read more]
    12 April, 2023
  • A modified kit-based HBV protein-free DNA extraction from liver tissues and cell cultures for HBV cccDNA Southern blot and qPCR

    • cccDNA quantification from total DNA preparation by qPCR with cccDNA-selective primers is challenging because of the coexisting HBV DNA forms (replicative intermediates, protein-free ... [Read more]
    21 September, 2022
  • A sensitive and rapid Southern blot assay based on branched DNA technology for the detection of HBV DNA in cell culture and liver tissue samples

    • Sothern blot is considered the “gold standard” for cccDNA detection because it can separate the cccDNA from protein-free relaxed circular DNA (pf-rcDNA, aka deproteinated rcDNA (DP-rc... [Read more]
    21 September, 2022
  • An Optimized Ex Vivo Flurospot assay to identify multi-functional HBV-specific T cells in Chronic Hepatitis B patients

    During a traditional FluroSpot assay, low HBV Specific T cell frequencies have hindered effective ex vivo analysis. We overcame this obstacle to measure ex vivo T cell responses in CHB patients, ... [Read more]
    07 March, 2022
  • Fluorescent-bait labelling for the ex vivo detection of HBV antigen-specific B cells

    Fluorescently conjugated antigen-bait systems have been extensively used to identify antigen-specific B cells and probe humoral immunity across different settings. Using this principle, HBV antig... [Read more]
    30 April, 2021
  • Tetramer enrichment for HBV-specific CD8+ T cells

    The frequency of HBV-specific CD8+ T cells can be very low, depending on the specificity. Therefore, they cannot always be detected by conventional ex vivo tetramer staining using 10[Read more]
    03 July, 2020
  • A fluorescent in situ hybridization (FISH) assay for detection of HBV DNA in cell culture models

    • Although a plethora of knowledge on the molecular life cycle of Hepatitis B Virus has been gained by utilizing classical methods, they can only inform on the average level of the test... [Read more]
    19 June, 2020
  • HBV Ribonuclease H assay resolved by denaturing PAGE

    • This assay characterizes the products of HBV RNase H activity on a DNA:RNA heteroduplex.
    • Products are resolved by denaturing PAGE to assess specificity of cleavage and eff... [Read more]
    06 June, 2019
  • HBV Ribonuclease H FRET Assay

    • This assay detects the activity of HBV RNaseH using fluorescence resonance energy transfer
    • The ribonuclease H (RNaseH) substrate is an RNA DNA heteroduplex in which an RNA... [Read more]
    06 June, 2019

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