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Author: admin

Abstract submission for ANRS HBV Cure workshop is open until April 21, 2017

POSTED ON: 15 Mar, 2017

 

Apply here.

Please note that the presentations are available as ‘posters’ only.

Deadlines:

Deadline for abstract submission: April 21st, 2017
Notification of acceptance:April 28th, 2017

 

 

Categories ANRS, Workshop

HBV Research Protocols

  • Tetramer enrichment for HBV-specific CD8+ T cells

    The frequency of HBV-specific CD8+ T cells can be very low, depending on the specificity. Therefore, they cannot always be detected by conventional ex vivo tetramer staining using 10[Read more]
    03 July, 2020
  • A fluorescent in situ hybridization (FISH) assay for detection of HBV DNA in cell culture models

    • Although a plethora of knowledge on the molecular life cycle of Hepatitis B Virus has been gained by utilizing classical methods, they can only inform on the average level of the test... [Read more]
    19 June, 2020
  • HBV Ribonuclease H assay resolved by denaturing PAGE

    • This assay characterizes the products of HBV RNase H activity on a DNA:RNA heteroduplex.
    • Products are resolved by denaturing PAGE to assess specificity of cleavage and eff... [Read more]
    06 June, 2019
  • HBV Ribonuclease H FRET Assay

    • This assay detects the activity of HBV RNaseH using fluorescence resonance energy transfer
    • The ribonuclease H (RNaseH) substrate is an RNA DNA heteroduplex in which an RNA... [Read more]
    06 June, 2019
  • A Southern Blot Assay for Detection of HBV cccDNA from Cell Cultures

    • As an essential component of HBV life cycle, the viral covalently closed circular DNA (cccDNA) is synthesized and maintained at low copy numbers in the nucleus of infected hepatocytes... [Read more]
    03 June, 2019
  • Modeling HBV infection and therapy in immunodeficient NOD-Rag1-/-IL2RgammaC-null (NRG) fumarylacetoacetate hydrolase (FAH) knockout mice with human chimeric liver

    • Besides human, only a few species have been reported to be permissive for Hepatitis B virus (HBV) infection(1-3). Human liver chimeric mouse models have been developed to overcome the... [Read more]
    25 May, 2019
  • Hepatitis B Virus Polymerase Epsilon RNA Binding Assays

    • HBV polymerase activity is assayed by measuring its ability to bind viral RNA at a secondary-structure motif, the epsilon (e) stem-loop structure.
    • HBV polymerase is expres... [Read more]
    24 May, 2019
  • In Vitro Hepatitis B Virus Polymerase Priming Assay

    • HBV polymerase activity is assayed by measuring the first step in DNA synthesis, protein priming, wherein the polymerase becomes nucleotide-labelled.
    • This in vitro[Read more]
    23 May, 2019
  • HBV and HDV infection in uPA/SCID mice with humanized livers

    • This protocol describes the infection of liver humanized mice (here UPA/SCID mice) with hepatitis B and /or hepatitis D viruses.
    • We also address the characteristics of infecti... [Read more]
    21 May, 2019
  • Evaluating mouse HBV-specific CD8+ T cell responses by ELISPOT assay

    • Immunocompetent mouse models that allow for the investigation of virus replication and the immune response to HBV include hydrodynamic injection, HBV genome transduction with adenovirus... [Read more]
    21 May, 2019

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ICE_HBVICE-HBV@ICE_HBV·
9 Apr

Helene is off to Mount Everest now to raise funds for #HBV cure research and show us this can be done despite #covid19 https://www.youtube.com/watch?v=f61pPyrVdZc #NoHep @OzCapucine

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AligosTheraAligos Therapeutics@AligosThera·
8 Apr

The Helene sur l'Everest project's proceeds will support the International Coalition to Eliminate HBV (@ICE_HBV), a @WHO-supported organization promoting global collaboration in research to support curative #hepatitisB treatments. Learn more about ICE-HBV: http://bit.ly/3skJFbk

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