HBV Research Protocols

There is an urgent need for centralized repositories of HBV-related materials that are readily accessible to HBV researchers globally. Critical to this will be quality assurance of the samples, and the availability of matching clinical data. This repository of HBV-related research protocols is designed to facilitate studies and the development of new drugs.

This project is designed to complement the upcoming NIAID reagents repository by making corresponding quality-controlled research protocols available freely for all researchers around the world.

When citing protocols from this database, please cite the original publications from which these protocols have been adapted. The origin of these original publications can be found within the protocols. Please also acknowledge this ICE-HBV Protocols Database. Any questions can be directed to info@ice-hbv.org

The review of these protocols has been led by Haitao Guo and the ICE-HBV working group members including Lena Allweiss, Maura Dandri, Jianming Hu, Jake Liang, Margaret Littlejohn, Peter Revill, and Barbara Testoni.

The development of the database is coordinated by Marley Easterbrook.

Immunofluorescent Staining for the Hepatitis B Core Antigen in frozen Liver Sections

Lena Allweiss, Marc Lütgehetmann, Maura Dandri

I. Deptartment of Internal Medicine, University Medical Center Hamburg-Eppendorf, Hamburg, Germany
  • The HBV core antigen (HBcAg) is the structural protein of the viral nucleocapsids but is also recruited to the nuclear HBV persistence form. Thus, HBcAg can be detected in the cytoplasm and nucleus of infected hepatocytes.
  • Here, we ... [Read more]

Stem Cell Derived Hepatocyte-Like Cells for Hepatitis B Virus Infection

Yuchen Xia (1), Seung Bum Park (2), T. Jake Liang (2)

1. Wuhan University, Wuhan, China 2. National Institutes of Health, Bethesda, USA
  • Human pluripotent stem cells derived hepatocyte-like cells (HLCs) are capable of expressing hepatocyte markers and host factors important for hepatic function maintenance.
  • These cells fully support HBV infection and virus-host interactions.
  • Stem cell-derived HLCs provide a new tool for antiviral drug screening and development.

Detection and characterisation of integrated Hepatitis B virus DNA using inverse nested PCR

Dr. Thomas Tu

Department of Infectious Diseases, Molecular Virology, University Hospital Heidelberg, Heidelberg, Germany
  • The integration of HBV DNA into the host cell genome can occur during HBV infection, though it does not produce new infectious virus particles.
  • HBV DNA integration is one possible cause of HBV-induced liver cancer and could b... [Read more]