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HBV Cure “Winter” Workshop
Recently Published Protocols
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HBV Ribonuclease H assay resolved by denaturing PAGE
- This assay characterizes the products of HBV RNase H activity on a DNA:RNA heteroduplex.
- Products are resolv... [Read more]
06 June, 2019 -
HBV Ribonuclease H FRET Assay
- This assay detects the activity of HBV RNaseH using fluorescence resonance energy transfer
- The ribonuclease ... [Read more]
06 June, 2019 -
A Southern Blot Assay for Detection of HBV cccDNA from Cell Cultures
- As an essential component of HBV life cycle, the viral covalently closed circular DNA (cccDNA) is synthesized and mainta... [Read more]
03 June, 2019 -
Modeling HBV infection and therapy in immunodeficient NOD-Rag1-/-IL2RgammaC-null (NRG) fumarylacetoacetate hydrolase (FAH) knockout mice with human chimeric liver
- Besides human, only a few species have been reported to be permissive for Hepatitis B virus (HBV) infection(1-3). Human ... [Read more]
25 May, 2019 -
Hepatitis B Virus Polymerase Epsilon RNA Binding Assays
- HBV polymerase activity is assayed by measuring its ability to bind viral RNA at a secondary-structure motif, the epsilo... [Read more]
24 May, 2019 -
In Vitro Hepatitis B Virus Polymerase Priming Assay
- HBV polymerase activity is assayed by measuring the first step in DNA synthesis, protein priming, wherein the polymerase... [Read more]
23 May, 2019 -
HBV and HDV infection in uPA/SCID mice with humanized livers
- This protocol describes the infection of liver humanized mice (here UPA/SCID mice) with hepatitis B and /or hepatitis D vi... [Read more]
21 May, 2019 -
Evaluating mouse HBV-specific CD8+ T cell responses by ELISPOT assay
- Immunocompetent mouse models that allow for the investigation of virus replication and the immune response to HBV include ... [Read more]
21 May, 2019 -
HBV RNA detection in liver tissues by in situ hybridization
- HBV RNA detection at the cellular level in the liver
- Multiplex ... [Read more]
21 May, 2019 -
Hydrodynamic HBV transfection in mouse models
- The method of hydrodynamic delivery (1, 2) was used to establish a mouse model of hepatitis B virus persistence by trans... [Read more]
13 May, 2019